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WIT-T Medium

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Cellaria’s WIT-T™ Culture Medium has been specifically optimized for the robust culture of human mammary epithelial cells of transformed phenotype (e.g., immortalized cells transformed either with SV40 early region, termed BPLE cells, or with both SV40 early region and H-ras, known as BPLER cells), an important experimental model for the study of tumor cell biology1-3. Transformed human mammary epithelial cells grown in WIT-T Culture Medium yield tumors that more closely resemble naturally occurring human adenocarcinomas than cells grown in other epithelial cell culture mediaa1,2.

  • Serum-free and chemically-defined culture environment
  • No feeder cells required
  • Fewer BPLER cells required for tumor formation
  • WIT-T supports highly tumorigenic and metastatic BPLER cells

Disease Area: Cancer

Tissue Type: Breast

Storage and Stability

Store basal medium at 4°C. Store supplement at -20°C. Media and supplement are stable for a minimum of 3 months from date of receipt, when stored as directed.

Quality Control

Breast primary epithelial cells are tested for proliferation and morphology during culture over three continuous passages. Product sterility is confirmed by negative test results for bacteria, fungi, and mycoplasma.

 

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