Cellaria’s WIT-T™ Culture Medium has been specifically optimized for the robust culture of human mammary epithelial cells of transformed phenotype (e.g., immortalized cells transformed either with SV40 early region, termed BPLE cells, or with both SV40 early region and H-ras, known as BPLER cells), an important experimental model for the study of tumor cell biology1-3. Transformed human mammary epithelial cells grown in WIT-T Culture Medium yield tumors that more closely resemble naturally occurring human adenocarcinomas than cells grown in other epithelial cell culture mediaa1,2.
- Serum-free and chemically-defined culture environment
- No feeder cells required
- Fewer BPLER cells required for tumor formation
- WIT-T supports highly tumorigenic and metastatic BPLER cells
Disease Area: Cancer
Tissue Type: Breast
Storage and Stability
Store basal medium at 4°C. Store supplement at -20°C. Media and supplement are stable for a minimum of 3 months from date of receipt, when stored as directed.
Breast primary epithelial cells are tested for proliferation and morphology during culture over three continuous passages. Product sterility is confirmed by negative test results for bacteria, fungi, and mycoplasma.
- Ince, T.A., Richardson, A.L., Bell, G.W., Saitoh, M., Godar, S., Karnoub, A.E., Iglehart, J.D., and Weinberg, R.A. (2007) Transformation of different human breast epithelial cell types leads to distinct tumor phenotypes. Cancer Cell 12: 160-170.
- Shay, J.W., and Wright, W.E. (2007) Tissue culture as a hostile environment: identifying conditions for breast cancer progression studies. Cancer Cell 12: 100-101.
- Godar, S., Ince, T.A, Bell, G.W., Feldser, D., Donaher, J.L., Bergh, J., Liu, A., Miu, K., Watnick, R.S., Reinhardt, F., McAllister, S.S., Jacks, T., and Weinberg, R.A. (2008) Growth-inhibitory and tumor-suppressive functions of p53 depend on its repression of CD44 expression. Cell 134: 62-73.
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